Complete description

Containing 57 thoroughly class-tested exercises, this manual provides basic microbiology techniques with applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Ninth Edition features a new, four-color design and a dramatically new art program. Many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style, and detailed, colorful photomicrographs that were once grouped together in a color insert are now integrated throughout the exercises. Experiments have been refined throughout, and a new exercise on parasitic helminths provides students with valuable practice in microscopic examination and observation. Top page

General info

Publisher & Imprint: Pearson

Edition details International ed of 9th revised ed

Pages: 496

More info: height 272 mm width 216 mm weight 980 gr thickness 13 mm

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Age recommended: College/higher education

Subject Indexing & Classification Dewey:(DC22) 579.078

Departments: Medical microbiology & virology;

Record updated at: 08 November, 2012 time: 17:27

Summary Laboratory Experiments in Microbiology Introduction I. MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms II. Handling Bacteria 3. Microbes in the Environment 4. Transfer of Bacteria: Aseptic Techniques III. STAINING METHODS 5. Preparation of Smears and Simple Staining 6. Negative Staining 7. Gram Staining 8. Acid-fast Staining 9. Structural Stains (Endospore, Capsule, Flagella) 10. Morphologic Unknown IV. CULTIVATION OF BACTERIA. 11. Isolation of Bacteria by Dilution Technique 12. Special Media for Isolating Bacteria V. MICROBIAL METABOLISM 13. Carbohydrate Catabolism 14. Fermentation 15. Protein Catabolism, Part 1 16. Protein Catabolism, Part 2 17. Respiration 18. Unknown Identification and Bergey's Manual VI. MICROBIAL GROWTH 19. Oxygen and the Growth of Bacteria 20. Determination of a Bacterial Growth Curve: The Role of Temperature 21. Biofilms VII. CONTROL OF MICROBIAL GROWTH 22. Physical Methods of Control: Heat 23. Physical Methods of Control: Ultraviolet Radiation 24. Chemical Methods of Control: Disinfectants and Antiseptics 25. Chemical Methods of Control: Antimicrobial Drugs 26. Effectiveness of Hand Scrubbing VIII. MICROBIAL GENETICS 27. Regulation of Gene Expression 28. Isolation of Bacterial Mutants 29. Transformation of Bacteria 30. DNA Fingerprinting 31. Genetic Engineering 32. Ames Test for Detecting Possible Chemical Carcinogens IX. THE MICROBIAL WORLD 33. Fungi: Yeasts and Molds 34. Phototrophs: Algae and Cyanobacteria 35. Protozoa 36. Parasitic Helminths X. VIRUSES 37. Isolation and Titration of Bacteriophages 38. Plant Viruses XI. INTERACTION OF MICROBE AND HOST 39. Epidemiology 40. Koch's Postulate XII. IMMUNOLOGY 41. Nonspecific Resistance 42. Blood Group Determination: Slide Agglutination 43. Agglutination Reactions: Microtiter Agglutination 44. ELISA Technique XIII. MICROORGANISMS AND DISEASE 45. Bacteria of the Skin 46. Bacteria of the Respiratory Tract 47. Bacteria of the Mouth 48. Bacteria of the Gastrointestinal Tract 49. Bacteria of the Genitourinary Tract 50. Rapid Identification Methods 51. Identification of an Unknown from a Clinical Sample XIV. MICROBIOLOGY AND THE ENVIRONMENT 52. Microbes in Water: Multiple-Tube Technique 53. Microbes in Water: Membrane Filter Technique 54. Microbes in Food: Contamination 55. Microbes Used in the Production of Foods 56. Microbes in Soil: The Nitrogen and Sulfur Cycles 57. Microbes in Soil: Bioremediaton APPENDICES A. Pipetting B. Dilution Techniques and Calculations C. Use of the Spectrophotometer D. Graphing E. Use of the Dissecting Membrane F. Use of the Membrane Filter G. Electrophoresis H. Keys to Bacteria Index Top page

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