Atlas of Human Pluripotent Stem Cells

Edited by Michal Amit - Joseph Itskovitz-Eldor

Atlas of Human Pluripotent Stem Cells

Edited by Michal Amit, Joseph Itskovitz-Eldor

Publisher: Humana Press Inc.

Series: Stem Cell Biology and Regenerative Medicine

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Format: Hardback

Publication date: 18 November 2011

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ISBN: 161779547X ISBN 13: 9781617795473

Atlas of Human Pluripotent Stem Cells

This comprehensive atlas offers step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions and in non-adhesion suspension culture, as well as methods for examining pluripotency and karyotype stability. Top page

Complete description

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells. Top page

General info

Publisher & Imprint: Humana Press Inc.

City: Totowa, NJ

Pages: 156

More info: height 235 mm width 155 mm weight 403 gr thickness 15 mm

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Age recommended: Professional and scholarly

Subject Indexing & Classification Dewey:(DC23) 616.02774 Library of Congress Subject: QH301-705 Life sciences

Departments: Medical research; Cellular biology; Genetics (non medical);

Record updated at: 14 August, 2014 time: 14:10

Summary Atlas of Human Pluripotent Stem Cells Methods for the Derivation of Human Embryonic Stem Cell Lines.- Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders.- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions.- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures.- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies.- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation.- Immunostaining.- Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines.- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes. Top page

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